Journal: Communications Biology

Article Title: Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosacea

doi: 10.1038/s42003-026-09662-3

Figure Lengend Snippet: A Schematic representation of establishing a mouse model of rosacea-like skin inflammation using LL37 and treatment with GBP (Created by BioRender); B Administration of GBP to different groups of mice and observation of skin symptoms at the end of the final experiment; C Assessment of each group of mice’s skin thickness; D Evaluation of skin redness score in each group of mice; E Overall skin condition score for each group of mice; F Histopathological assessment using H&E staining to examine skin conditions of each group of mice, with a scale bar of 100 μm and a local magnification scale bar of 25 μm; G Analysis of inflammatory cell infiltration in different groups. All data are expressed as mean ± standard deviation (SD). Comparisons among multiple groups were performed using one-way analysis of variance (ANOVA), followed by post-hoc tests with Dunnett’s T3 and LSD-t methods. For animal experiments, n = 5. Statistical significance was defined as ns P > 0.05; *** P < 0.001.

Article Snippet: Anesthetized with pentobarbital sodium (50 mg/kg), the mice were subcutaneously injected with 40 μL of 320 μM LL37 (HY-P1222, MCE) twice a day for two days, wild-type mice were used as controls.

Techniques: Staining, Standard Deviation

Journal: Communications Biology

Article Title: Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosacea

doi: 10.1038/s42003-026-09662-3

Figure Lengend Snippet: A Collection of skin tissue from various groups of mice for transcriptome high-throughput sequencing (Created by BioRender); B Volcano plot analysis of DEGs between the Ctrl group and LL37 group from high-throughput transcriptome sequencing, where blue dots represent downregulated genes, red dots depict upregulated genes, and gray dots indicate insignificant genes, n = 3; C Differential gene analysis between LL37 and GBP groups by high-throughput transcriptome sequencing, with blue dots representing downregulated genes, red dots indicating upregulated genes, and gray dots showing insignificant genes, n = 3; D PCA analysis of differential genes between Ctrl, LL37, and GBP groups; E Venn diagram analysis of differential genes between Ctrl, LL37, and GBP groups, with blue circle representing genes differentially expressed in Ctrl and LL37 groups, and red circle indicating genes differentially expressed in LL37 and GBP groups; F Heat map showing the expression of selected inflammatory factors in the intersecting genes.

Article Snippet: Anesthetized with pentobarbital sodium (50 mg/kg), the mice were subcutaneously injected with 40 μL of 320 μM LL37 (HY-P1222, MCE) twice a day for two days, wild-type mice were used as controls.

Techniques: Next-Generation Sequencing, High Throughput Screening Assay, Sequencing, Expressing

Journal: Communications Biology

Article Title: Transcriptomic and metabolomic insights into gabapentin’s therapeutic role in neurogenic inflammation of rosacea

doi: 10.1038/s42003-026-09662-3

Figure Lengend Snippet: A KEGG and GO analyses reveal the upregulated DEGs controlling biological pathways in the LL37 and GBP groups; B KEGG and GO analyses show the downregulated DEGs controlling biological pathways in the LL37 and GBP groups; C , D GSEA analysis indicates the expression pattern of inflammatory factors regulated by Neuroinflammation and Glutamatergic Signaling; E , F GSEA analysis shows the expression pattern of inflammatory factors regulated by the NF-κB Signaling pathway.

Article Snippet: Anesthetized with pentobarbital sodium (50 mg/kg), the mice were subcutaneously injected with 40 μL of 320 μM LL37 (HY-P1222, MCE) twice a day for two days, wild-type mice were used as controls.

Techniques: Expressing

Journal: MedComm

Article Title: Outer membrane vesicle contributes to the Pseudomonas aeruginosa resistance to antimicrobial peptides in the acidic airway of bronchiectasis patients

doi: 10.1002/mco2.70084

Figure Lengend Snippet: Lactate‐mediated acidification dampens the bactericidal activity of LL‐37 against P. aeruginosa . (A) Lactate concentrations of the sputa were collected from bronchiectasis (BE) patients and their relatives as controls in cohort 3. Induced sputa were collected when needed. The comparison was performed with an unpaired Student t ‐test. (B) Comparison of the sputum LL‐37 concentrations between BE patients with different microbiology test results in cohort 3. A comparison was performed with one‐way analysis of variance (ANOVA) with Welch's test. (C) The lactate concentrations in bronchoalveolar lavage fluid (BALF) from mice with or without P. aeruginosa chronic lung infection. A comparison was performed with an unpaired Student t ‐test. (D) The survivals of three clinical P. aeruginosa isolated from BE patients after the treatment with LL‐37 in vitro. Comparison between the untreated and LL‐37‐treated groups (120 µg/mL) was performed with an unpaired Student t ‐test. (E) Percentages of survived clinical P. aeruginosa isolate SHPHC2 post‐LL‐37 treatment in the conditions with various pH adjusted by lactate. One‐way ANOVA with Welch's test was used for statistical analysis in both LL37‐treated and untreated groups, p ‐values were indicated in the figure, and results with a p ‐value less than 0.05 were considered statistically significant. The error bars plotted indicate the standard error of the mean.

Article Snippet: The final concentrations of LL‐37 (Tocris Bioscience, cat. 5213), P. aeruginosa ‐derived OMVs, HHQ (TargetMol, cat. T19713), PQS (TargetMol, cat. T38373) and Cl‐adimine (MedChemExpress, cat. HY‐100574) were stated in the main text.

Techniques: Activity Assay, Comparison, Infection, Isolation, In Vitro

Journal: MedComm

Article Title: Outer membrane vesicle contributes to the Pseudomonas aeruginosa resistance to antimicrobial peptides in the acidic airway of bronchiectasis patients

doi: 10.1002/mco2.70084

Figure Lengend Snippet: P. aeruginosa produces outer membrane vesicles (OMVs) to resist LL‐37‐mediated killing. (A) Quantification of OMVs produced by P. aeruginosa in the lactate‐mediated acidic environment by measuring FM 4‐64 fluorescence. One‐way analysis of variance (ANOVA) with Welch's test was used for statistical analysis. (B) Nanoparticle tracking analysis and transmission electron microscopy of OMVs isolated from P. aeruginosa overnight culture by ultracentrifugation. (C) Survival of P. aeruginosa PAO1 post‐LL‐37 treatment with the additions of OMVs of different dosages. The OMVs added were quantified by measuring the total proteins enwrapped. One‐way ANOVA with Welch's test was used for statistical analysis in both LL37‐treated and untreated groups and the p ‐value was shown. (D) The role of OMVs in the acidic condition‐induced strengthening of P. aeruginosa LL‐37 resistance was determined by adding Cl‐adimine to the bacteria‐killing system. The final concentration of Cl‐diamine was indicated on the diagram. The differences between groups were statistically analyzed by using one‐way ANOVA with Welch's test, with the p values of multiple comparisons indicated in the figures. Results with a p ‐value less than 0.05 were considered statistically significant. The error bars plotted indicate the standard error of the mean.

Article Snippet: The final concentrations of LL‐37 (Tocris Bioscience, cat. 5213), P. aeruginosa ‐derived OMVs, HHQ (TargetMol, cat. T19713), PQS (TargetMol, cat. T38373) and Cl‐adimine (MedChemExpress, cat. HY‐100574) were stated in the main text.

Techniques: Membrane, Produced, Fluorescence, Transmission Assay, Electron Microscopy, Isolation, Bacteria, Concentration Assay